The analysis of nucleic acids, their characterization gets more and more important nowadays. Beside forensic and ancestry matters, medical diagnostic and specialized treatment in therapy are the driving forces. Verifying the structure of synthesized medicine, its sequence is one task to cover.
Synthesis Confirmation for Nucleic Acid Medicines - Rapid Sequence Confirmation Using a MALDI-TOF Mass Spectrometer
Medicines utilizing nucleic acids such as DNA and RNA that control genetic information are called "nucleic acid medicines". These nucleic acid medicines allow targeting of molecules such as messenger RNA (mRNA) and micro RNA (miRNA) which cannot be targeted with traditional low-molecular-weight drugs and antibody medicines, and are expected to be innovative next generation pharmaceuticals for the treatment of genetic disorders which have been difficult to treat so far.
Mass spectrometry has the capacity to analyze oligonucleotides more quickly and accurately than many other techniques. These important factors become critical in a high throughput environment, for example in the case of industrial primer synthesis where tens of thousands of samples may be generated each day. Here, it is vital that the product quality is of a high standard and that the correct sequence has been generated. Limitations arise when the nature of the sample is considered. For example, electrospray experiments may be difficult due to the high salt content of synthesized primers that can suppress the ionization process, produce cationic salt adducts, and promote multiple charge states making spectral interpretation more complicated.
Determination of Molecular Mass and Quantification of Oligonucleotide Therapeutics Using Quadrupole Time-of-Flight Mass Spectrometer LCMS™-9030
Oligonucleotide therapeutics are synthetic oligonucleotides that demonstrate their medical efficacy through binding to target genes or target proteins that may be responsible for a range of diseases. To date, eight types of oligonucleotide therapeutics have been approved, many of which have a length of approximately 20 bases. As an oligonucleotide therapeutic, the 2’-MOE modified oligonucleotide having 20 bases was used.
Convenient and Quick Isolation and Analysis of Genome-Edited Cells Using PERFLOW Sort and MultiNA
With the growing popularity of genome editing techniques, a major concern for researchers is: how efficiently intended edits can be achieved on cells or organisms, and how the type of editing made can be identified by a high-throughput process. These processes of concern, however, are described in papers as if they are very simple procedures. For example, in a paper which describes established genome-edited cells and their characterization analysis, the establishment of a mutated cell line is often summarized in a sentence like the one indicated below (the description about the transfection amount and incubation time is omitted). Genome-editing tools were delivered by the ** method, and single-cell cloning was performed to obtain ** and ** clones of heterozygously and homozygously mutated cells, respectively.
Three-Dimensional Spectra Measurement of Fluorescent Probes used for DNA Detection
DNA probes labeled with fluorescent dye (below referred to as fluorescent probes) are used extensively to detect and identify specific DNA when conducting life science studies. The mechanism involves the selective binding of the probe to specific DNA, thereby permitting the detection of that DNA. However, due to the wide variety of fluorescent dyes, it is important to know the exact wavelength at which the probe fluoresces to ensure DNA detection. Here, using the three-dimensional spectral measurement feature of the RF-6000 Spectrofluorophotometer, we introduce examples of fluorescence measurement of two types of fluorescent probes.
Quantitation of dsDNA Using the Micro-Volume BioSpec-nano Spectrophotometer
The Shimadzu BioSpec-nano is a low-maintenance micro-volume spectrophotometer designed for the modern life science laboratory. It offers superior detection limits, up to 10 times better compared to the competition, making it the perfect instrument for quantitation of DNA, RNA, Protein analysis, and photometric measurements. The “Drop and Click” design combined with easy sample mounting and automated cleaning offers a rapid 3 second analysis time and a 10 second cycle time between samples.