Cell Culture & Cell Culture Media Analysis
Cell culture is a method to study the behavior of cells in a controlled environment, free of systematic variations. Different types have been adapted and developed based on the purpose of the investigation, such as interactions of drugs and other chemicals with cells, production of vaccines and proteins, e.g.
To care for optimal conditions, the composition of the media is checked regularly, therefore different chemical compounds and parameters are controlled, different techniques and applications are used.
One of such specialized solution is the C2MAP system. You can find out more details about the system here.
More applications and reports
A Novel Cell Culture Media Analysis Platform for Culture Process Development
Optimization and control of cell culture processes are essential to increase production efficiency of biopharmaceuticals. In the field of cell therapy including regenerative medicines, enhanced control of the culture process is also becoming important to reduce cell variability and improve consistency of mass production of the cells. Comprehensive monitoring of culture supernatant components gives researchers useful information for these purposes. However, current technologies for process monitoring are limited to measurement of pH, dissolving gases, and some small compounds such as glucose, glutamine, lactate, and ammonia in culture supernatant. We have developed a “Cell Culture Media Analysis Platform, C2MAP system” that combines automated pretreatment module for culture supernatant samples with LC/MS/MS.
Evaluation of Undifferentiated State of Human iPS Cells using C2MAP Cell Culture Media Analysis Platform
Development of technologies for the preparation and supply of iPS cells with high quality and a large amount is an essential requirement for commercialization of regenerative medicine. Conventionally, manual techniques such as Gene Expression analysis had been used to evaluate the status of cell differentiation, but because these are invasive techniques that cause cell disruption, they were generally applied as evaluation methods after culturing was completed.
Comprehensive Cell Culture Profiling Using the LCMS-9030 Quadrupole TOF Mass Spectrometer
In the production of useful substances by fermentation and the manufacturing of antibody drugs, the monitoring of culture media pH, dissolved gases, carbon sources (glucose), and nitrogen sources (glutamine) is implemented in order to optimize and manage the cultivation process. In addition to glucose and glutamine, the culture media components in cell cultures comprise vitamins and nucleic acid related compounds as well as metabolites and other compounds secreted from cells. Accordingly, the comprehensive analysis of compounds contained culture media is expected to provide useful information for discussing bioprocesses.
Simple Evaluation of Cultured Cells: Evaluation of Differentiation State of iPS Cells Using Benchtop MALDI-TOF MS
The growth condition and differentiation state (differentiated or undifferentiated) of cultured cells are important evaluation items in regenerative medicine and basic research. If these cell evaluations can be conducted in a simple manner by mass spectrometry, evaluation time and complicated work can be significantly reduced. Therefore, in order to examine the possibility of determining the state of cells by mass spectrometry, we conducted an automatic analysis of iPS cells and their culture media by AuraSolution™ using the Shimadzu MALDI-8020 benchtop linear MALDI-TOF mass spectrometer, and attempted a preliminary analysis by the statistical analysis software eMSTAT Solution™.
Evaluation of Differentiated/Undifferentiated State of iPS Cells Using DPiMS-2020 and eMSTAT Solution
This article introduces an example of quick and simple measurement of the components contained in iPS cell culture medium with a DPiMS-2020 mass spectrometer using the probe electro spray ionization method, which is a new ionization method. This article also introduces an example in which grouping of the culture media of differentiated/undifferentiated cells was possible by using eMSTAT Solution statistical analysis software.